2003, English, Data set edition: North West Shelf, June 2003, (RV Melville voyage), HPLC Pigment and Ocean Colour Data

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Physical Description
  • data set
Published
  • Australian Ocean Data Network
Language
  • English

Edition details

Title
  • North West Shelf, June 2003, (RV Melville voyage), HPLC Pigment and Ocean Colour Data
Other Contributors
  • CSIRO Oceans and Atmosphere - Information and Data Centre (pointOfContact)
Published
  • Australian Ocean Data Network
Physical Description
  • data set
Part Of
  • http:/​/​www.marlin.csiro.au
Subjects
Summary
  • Water samples were taken on-board the RV Melville and stored under cool and dark conditions until filtering took place. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory, CMR, Hobart. Pigment analysis; 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/​F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop.Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption; 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/​F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/​VIS dual beam spectrophotometer equipped with an integrating sphere
  • This record contains data collected on the RV Melville between 18 and 24 June 2003 in the North West Shelf. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and the absorption coefficient for particulate (ap) and detrital or non-algal (ad) components of the water column. 29 stations were sampled as well as 30 underway locations. The data was used primarily to validate ocean colour sensors MERIS, MODIS and SeaWIFs and the SST sensor AATSR.
  • Woods Hole Institute of Oceanography
  • Lesley Clementson
Language
  • English
Identifier
  • oai:ands.org.au::692064
  • ddbf0f84-36f7-3f5e-e043-08114f8cb5b0

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