English, Article edition: DISPERSION AND FIXATION OF ADENO-ASSOCIATED VIRUS WITH GLUTARALDEHYDE FOR AFM STUDIES PENG WANG; XINYAN WANG; HAIJUN YANG; ...

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Bookmark: http://trove.nla.gov.au/version/84781
Physical Description
  • article
Language
  • English

Edition details

Title
  • DISPERSION AND FIXATION OF ADENO-ASSOCIATED VIRUS WITH GLUTARALDEHYDE FOR AFM STUDIES
Author
  • PENG WANG
  • XINYAN WANG
  • HAIJUN YANG
  • JUNHONG LÃ
Physical Description
  • article
Notes
  • Sample preparation is an important procedure for atomic force microscope (AFM) studies. However, flexible virus particles have a tendency to aggregate together and are easily compressed during sample preparation or by AFM tip that subsequently hamper studying of virus by AFM. Herein, low concentration chemical reagent of glutaraldehyde (2%, v/​v) is pre-mixed in virus suspension that facilitates the dispersion and observation of recombinant serotype 2 adeno-associated virus particles deposited on mica surface with little deformation.
  • Adeno-associated virus (AAV), atomic force microscope (AFM), glutaraldehyde, dispersion, fixation
  • RePEc:wsi:srlxxx:v:15:y:2008:i:05:p:595-598
Language
  • English
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