2006-11-01, English, Article, Journal or magazine article edition: Silver staining of proteins in polyacrylamide gels. Chevallet, Mireille; Luche, Sylvie; Rabilloud, Thierry

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Physical Description
  • 5897 words
  • Magazine/​Journal article
Published
  • Nature Publishing Group, 2006 Nov
Language
  • English

Edition details

Title
  • Silver staining of proteins in polyacrylamide gels.
Appears In
  • Nature Protocols, v.1, no.4, 2006 Nov, p.1852(7) (ISSN: 1754-2189)
Author
  • Chevallet, Mireille
  • Luche, Sylvie
  • Rabilloud, Thierry
Published
  • Nature Publishing Group, 2006 Nov
Physical Description
  • 5897 words
  • Magazine/​Journal article
Subjects
Target Audience
  • Academic
Summary
  • Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. It is compatible with downstream processing, such as mass spectrometry analysis after protein digestion. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation and finally image development. Several variants of silver staining are described here, which can be completed in a time range from 2 h to 1 d after the end of the electrophoretic separation. Once completed, the stain is stable for several weeks.
Terms of Use
  • COPYRIGHT 2006 Nature Publishing Group
Language
  • English
Database
  • Gale Academic OneFile
Identifier
  • 182037388

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