Antagonistic activity of probiotic bacteria based on bacterial diversity in the porcine gut / Sameer M. Dixit Dixit, Sameer M

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Author
Dixit, Sameer M
Subjects
University of Western Sydney, Hawkesbury; Swine - Diseases.; Escherichia coli infections in swine.
Summary
Diversity analysis of Escherichia coli have routinely utilised isolates obtained by culture of faeces on MacConkey selective media, under the assumption that the diversity identified in faecal isolates are representative of similar diversity in E. coli in the gastrointestinal tract (GIT). This study has addressed this important issue by specifically isolating E. coli from different regions of the gut in pigs and subjecting them to enzymatic multilocus enzyme electrophoresis (MLEE) and molecular virulence factor (VF) analysis to ascertain whether E. coli populations inhabiting different regions of the gut are different from each other. Combination of these results showed that on average, E. coli strains isolated from the upper GIT region (small intestine) of the pig are distinctly different from the E. coli strains isolated from the lower GIT region (large intestine). An important aspect of the finding that faecal E. coli are not truly representative of the diversity in the GIT is the mechanism used by specific clonotypes that have adapted to different geographical habitats to survive challenge from incoming strains. FULL ACCESS RESTRICTED UNTIL 25/04/2021. Doctor of Philosophy (PhD)
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http://trove.nla.gov.au/work/3905299
Work ID
3905299

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