1993, English, Article edition: Construction of multiple copy of {alpha}-domain gene fragment of human liver metallothionein IA in tandem arrays and its expression in transgenic tobacco plants Pan, Aihua; Tie, Feng; Yang, Meizhu; ...

User activity

Share to:
 
Bookmark: http://trove.nla.gov.au/version/210532
Physical Description
  • text/​html
Published
  • Oxford University Press
  • 1993-09-01 00:00:00.0
Language
  • English

Edition details

Title
  • Construction of multiple copy of {alpha}-domain gene fragment of human liver metallothionein IA in tandem arrays and its expression in transgenic tobacco plants
Author
  • Pan, Aihua
  • Tie, Feng
  • Yang, Meizhu
  • Luo, Jingchu
  • Wang, Zhengxing
  • Ding, Xiang
  • Li, Lingyuan
  • Chen, Zhangliang
  • Ru, Binggen
Published
  • Oxford University Press
  • 1993-09-01 00:00:00.0
Physical Description
  • text/​html
Subjects
Notes
  • Metallothioneins (MT) are low molecular weight, cysteinerich, metal-binding proteins. An MT molecule contains two domains which appear to act independently—an α-domain, which is characterized by cadmium-binding, and a β-domain, which binds preferentially to copper. Based on this conception, DNA duplex encoding the α-domain (106 bp) of human MT-I A was constructed from a chemically-synthesized oligomer by repair synthesis and enzymatic ligation and cloned into pUC19. The genes cloned were sequenced and found to be in the correct order as designed. Synthetic directional adapters were attached to the terminals of the α-domain gene fragment of human MT-I A to establish complete control over fragment orientation during ligation. The use of these directional adapters thereby ensured the production of multiple copies of the α-domain in tandem arrays. The successive α-domains were linked by a peptide linker consisting of 10 residues. A chimeric gene containing 12 cloned tandemly repeated copies of the 106 bp α-domain DNA was introduced into tobacco cells on a disarmed Ti-plasmid of Agrobacterium tumefaciens . A total of 10 different transgenic tobacco plants were generated, of which two showed root and shoot growth unaffected by up to 200 mg&​sol;l kanamycin and 100 μM cadmium, whereas root growth of control plants was severely inhibited and leaf chlorosis developed on media containing only 10 μM cadmium
Terms of Use
  • Copyright (C) 1993, Oxford University Press
Language
  • English
Contributed by
OAIster

Get this edition

With access conditions

  • Set up My libraries

    How do I set up "My libraries"?

    In order to set up a list of libraries that you have access to, you must first login or sign up. Then set up a personal list of libraries from your profile page by clicking on your user name at the top right of any screen.

  • All (1)
  • Unknown (1)
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.

User activity


e.g. test cricket, Perth (WA), "Parkes, Henry"

Separate different tags with a comma. To include a comma in your tag, surround the tag with double quotes.

Be the first to add a tag for this edition

Be the first to add this to a list

Comments and reviews

What are comments? Add a comment

No user comments or reviews for this version

Add a comment