2006, English, Article edition: Purification of tubulin from limited volumes of cultured cells. Fourest-Lieuvin, Anne

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Physical Description
  • text
Published
  • HAL - CCSD
  • 2006
Language
  • English

Edition details

Title
  • Purification of tubulin from limited volumes of cultured cells.
Author
  • Fourest-Lieuvin, Anne
Published
  • HAL - CCSD
  • 2006
Physical Description
  • text
Subjects
Notes
  • A method was designed to purify tubulin from limited volumes of cultured cells, which can be performed in less than 4 h. The method is based on the preservation of intact microtubule arrays during cell lysis in a large volume of buffer, followed by disassembly of microtubules in a small volume of cold buffer. This allows a good enrichment in tubulin, which is then purified by one cycle of polymerisation/​depolymerisation and a cation exchange chromatography. Such a procedure has been employed successfully on suspension-cultured and on adherent HeLa cells. Tubulin obtained was 90% pure, assembly-competent and composed of alpha/​beta I and alpha/​beta IV isotypes. Microtubules made with this tubulin displayed specific properties such as resistance to dilution, maybe related to their specific dynamic behaviour.
  • HAL:inserm-00380060, version 1
  • HAL:http:/​/​www.hal.inserm.fr/​inserm-00380060/​en/​
  • PUBMED:16027005
  • DOI:10.1016/​j.pep.2005.05.011
Language
  • English
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