1993, English, Article edition: Cloning and Sequencing of cDNA for Glycolate Oxidase from Pumpkin Cotyledons and Northern Blot Analysis Tsugeki, Ryuji; Hara-Nishimura, Ikuko; Mori, Hitoshi; ...

User activity

Share to:
 
Bookmark: http://trove.nla.gov.au/version/201768
Physical Description
  • text/​html
Published
  • Oxford University Press
  • 1993-01-01 00:00:00.0
Language
  • English

Edition details

Title
  • Cloning and Sequencing of cDNA for Glycolate Oxidase from Pumpkin Cotyledons and Northern Blot Analysis
Author
  • Tsugeki, Ryuji
  • Hara-Nishimura, Ikuko
  • Mori, Hitoshi
  • Nishimura, Mikio
Published
  • Oxford University Press
  • 1993-01-01 00:00:00.0
Physical Description
  • text/​html
Subjects
Notes
  • A cDNA clone for glycolate oxidase (EC 1.1.3.1) was isolated by an immunochemical method from acDNA expression library constructed from poly(A)+-RNA of green pumpkin cotyledons. The analysis of in vitro transcription-translation products of the cDNA insert revealed that the cDNA clone contained the complete coding region for glycolate oxidase. The entire insert of the cDNA was 1,440 nucleotides in length and encoded 367 amino acid residues, equivalent to a molecular mass of 40,353 daltons. The amino acid sequence of the C-terminal tripeptide was Pro-Arg-Leu, which is slightly different from the proposed signal for targeting to microbodies, Ser-Lys/​Arg/​His-Leu. Characteristic hydrophilic domains observed in the C-terminal regions of most microbody proteins were found in the deduced sequence of glycolate oxidase by hydropathy analysis. Immunoblot analysis showed that the amount of glycolate oxidase was low in dark-grown cotyledons and increased during greening of pumpkin cotyledons. Northern blot analysis showed that the probe could hybridize with a single 1.5-kb species of mRNA from pumpkin cotyledons and that the amount of the hybridizable mRNA increased dramatically during greening of the cotyledons. This observation indicates that the induction of glycolate oxidase during greening of the cotyledons is due to an increase in the level of the mRNA.
Terms of Use
  • Copyright (C) 1993, The Japanese Society of Plant Physiologists
Language
  • English
Contributed by
OAIster

Get this edition

With access conditions

  • Set up My libraries

    How do I set up "My libraries"?

    In order to set up a list of libraries that you have access to, you must first login or sign up. Then set up a personal list of libraries from your profile page by clicking on your user name at the top right of any screen.

  • All (1)
  • Unknown (1)
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.

User activity


e.g. test cricket, Perth (WA), "Parkes, Henry"

Separate different tags with a comma. To include a comma in your tag, surround the tag with double quotes.

Be the first to add a tag for this edition

Be the first to add this to a list

Comments and reviews

What are comments? Add a comment

No user comments or reviews for this version

Add a comment