1993, English, Article edition: Preparation of a Monoclonal Antibody Specific for a 48-kDa Protein from Mitochondrial Nucleoids of the Yeast, Saccharomyces cerevisiae Miyakawa, Isamu; Tokitaka, Masaaki; Shiiba, Daisuke; ...

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Physical Description
  • text/​html
Published
  • Oxford University Press
  • 1993-01-01 00:00:00.0
Language
  • English

Edition details

Title
  • Preparation of a Monoclonal Antibody Specific for a 48-kDa Protein from Mitochondrial Nucleoids of the Yeast, Saccharomyces cerevisiae
Author
  • Miyakawa, Isamu
  • Tokitaka, Masaaki
  • Shiiba, Daisuke
  • Sawada, Tomoo
  • Fujikura, Yoshihisa
  • Fukumoto, Tetsuo
  • Sando, Nobundo
Published
  • Oxford University Press
  • 1993-01-01 00:00:00.0
Physical Description
  • text/​html
Subjects
Notes
  • Monoclonal antibodies (mAbs) were raised against yeast mitochondrial nucleoids (mtnucleoids). In an analysis by a combination of immunofluorescence microscopy and staining with 4′,6-diamidino-2-phenylindole (DAPI), one of them, designated YMN-1, distinctly stained mtnucleoids, which were visible as dots, in spheroplasts and in isolated mitochondria. However, staining of isolated mt-nucleoids was rather weak. YMN-1 mAb recognized a 48-kDa protein in immunoblots of both mitochondrial and mt-nucleoid proteins. The 48-kDa protein was a minor component of mt-nucleoid proteins and was separated from extract of both mitochondria and mt-nucleoids by immunoamnity chromatography. The affinity-purified 48-kDa protein reassociated with mt-nucleoids when mixed with isolated mt-nucleoids, as monitored by immunofluorescence microscopy. The results suggest that a large amount of 48-kDa protein is associated with mt-nucleoids in vivo, and that lysis of mitochondria by the treatment with detergent releases a considerable amount of this protein from mt-nucleoids during the isolation of mt-nucleoids.
Terms of Use
  • Copyright (C) 1993, The Japanese Society of Plant Physiologists
Language
  • English
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