1993, English, Article edition: Presence of a Pathway for the Biosynthesis of Auxin via Indole-3-Acetamide in Trifoliata Orange Kawaguchi, Masayoshi; Fujioka, Shozo; Sakurai, Akira; ...

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Physical Description
  • text/​html
Published
  • Oxford University Press
  • 1993-01-01 00:00:00.0
Language
  • English

Edition details

Title
  • Presence of a Pathway for the Biosynthesis of Auxin via Indole-3-Acetamide in Trifoliata Orange
Author
  • Kawaguchi, Masayoshi
  • Fujioka, Shozo
  • Sakurai, Akira
  • Yamaki, Yoshikazu T.
  • Syono, Kunihiko
Published
  • Oxford University Press
  • 1993-01-01 00:00:00.0
Physical Description
  • text/​html
Subjects
Notes
  • The auxin-biosynthetic pathway from L-tryptophan to indole-3-acetic add via indole-3-acetamide (IAM), found in plant-pathogenic bacteria such as Agrobacterium tumefaciens and Pseudomonas savastanoi , has not been found in plants. We attempted to detect the enzymatic activities for this pathway in cell-free systems from various tissues of trifoliata orange ( Poncirus trifoliata Rafin.). A high level of activity of LAM hydrolase, which catalyzes the conversion of IAM to indole-3-acetic acid, was observed in a crude extract prepared from young fruits one week after full bloom. Using α-naphthaleneacetamide as a competitor of IAM hydrolase, a simple assay system was developed for the detection of the conversion of L-tryptophan to IAM (tryptophan monooxygenase activity). When this system was used to assay cell-free extracts of young fruit of P. trifoliata , the conversion of L-tryptophan to IAM was clearly demonstrated by the presence of IAM among reaction products, as demonstrated by GC/​MS analysis and the incorporation of 14C-labeled L-tryptophan into an IAM fraction. This is the first report indicating the presence of an auxin-biosynthetic pathway via IAM in P. trifoliata . Furthermore, it is shown that both enzyme activities in auxin biosynthesis increased transiently during fruit development.
Terms of Use
  • Copyright (C) 1993, The Japanese Society of Plant Physiologists
Language
  • English
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