Carotenoids accumulation and expression of carotenogenesis genes during seedling and leaf development in Chinese cabbage ('Brassica rapa subsp. pekinensis') Tuan, Pham Anh; Park, Nam Il; Park, Woo Tae; ...

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Authors
Tuan, Pham Anh ; Park, Nam Il ; Park, Woo Tae ; Kim, Yeon Bok ; Kim, Jae Kwang ; Lee, Jonghoon ; Lee, Suh-hee ; Yang, Tae-Jin ; Park, Sang Un
Appears In
Plant OMICS
Subjects
Chinese cabbage -- Genetic aspects; Chinese cabbage -- Physiological aspects; Seedlings -- Physiological aspects
Audience
Academic
Summary
To investigate the regulation mechanisms of carotenoid biosynthesis in Chinese cabbage (Brassica rapa subsp. pekinensis), carotenoids accumulation and carotenogenesis genes (BrPSY, BrPDS, BrZDS, BrLCYB, BrLCYE, BrCHXB, BrZEP, and BrNCED) involved in carotenoids biosynthesis were analyzed during seedling and leaf development using real-time PCR. For seedling, some of carotenogenesis genes were showed similar to expression pattern between under dark and light condition. Carotenogenesis genes reached the maximum transcription at 8 days after sowing and this was similar to the highest contents of most detected carotenoids. Carotenoids contents (mainly lutein and [beta]-carotene) in light-grown seedlings were several time higher than those in dark-grown ones. For leaf development, direct lighted-outer leaves contain a 30-fold higher carotenoid amount compared to inner and middle leaves. However, expression level of carotenogenesis genes in outer leaves did not show a big different with those in inner and middle leaves. These results suggest a considerable role of light in carotenoids biosynthesis during seedling and leaf development. Keywords: Carotenoid, Brassica rapa, Chinese cabbage, carotenogenesis genes, gene expression. Abbreviations: DEPC, diethylpyrocarbonate; DW, dry weight; HPLC, high-performance liquid chromatography; DAS, days after sowing; GGDP, geranylgeranyl diphosphate; PSY, phytoene synthase; PDS, phytoene desaturase; ZDS, [xi]-carotene desaturase; LCYB, lycopene [beta]-cyclase; LCYE, lycopene [epsilon]-cyclase; CHXB, [beta]-ring carotene hydroxylase; CHXE, [epsilon]-ring carotene hydroxylase; ZEP, zeaxanthin epoxidase; NCED, 9-cis epoxycarotenoid dioxygenase; I-leaves, inner leaves; M-leaves, middle leaves; O-leaves, outer leaves.
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http://trove.nla.gov.au/work/165365415
Work ID
165365415

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