2012, Eng, eng , Thesis edition: Modifier genes in Lynch syndrome: functional genomics and its consequence on disease expression Reeves, Stuart

User activity

Send to:
 
Bookmark: http://trove.nla.gov.au/version/180226201
Physical Description
  • thesis
Published
  • 2012
Language
  • Eng
  • eng

Edition details

Title
  • Modifier genes in Lynch syndrome: functional genomics and its consequence on disease expression
Author
  • Reeves, Stuart
Other Contributors
  • University of Newcastle. Faculty of Health, School of Biomedical Sciences and Pharmacy
Published
  • 2012
Physical Description
  • thesis
Subjects
Summary
  • Research Doctorate - Doctor of Philosophy (PhD)
  • Colorectal cancer (CRC) is globally a major cause of morbidity and mortality. Each year more than one million patients will be diagnosed with colorectal cancer, with about 15 - 20% of these patients having a family history or an inherited colorectal cancer syndrome. Somewhere between 1% and 7% (dependent on population under study) of these cases will have Lynch syndrome, which is the most common hereditary autosomal-dominant inherited cancer syndrome caused by germline mutations in deoxyribonucleic acid (DNA) mismatch repair genes. Patients diagnosed with Lynch syndrome who harbour a confirmed germline mutation in DNA mismatch repair (MMR) genes have an 80% lifetime risk of developing an epithelial malignancy. Each patient belongs to a family that requires special medical attention including genetic counselling, DNA testing for mismatch repair genes (most frequently hMLH1 or hMSH2) and screening for CRC. There is, however, considerable variation in the age of disease onset which is explained by a combination of genetic and environmental factors. The studies described in this thesis are aimed to better understand the genetic modifying effects on disease expression and how they relate to the likely age of colorectal cancer onset. Previous studies have identified a polymorphic CA repeat region in IGF-1 and two specific single nucleotide polymorphisms in MTHFR that were thought to alter the age of disease onset in individuals with Lynch syndrome. The effects of these polymorphisms were examined in larger multinational cohorts of patients and found to have significant effects on disease onset age. This is discussed in chapters 2, 3 and 4. Another similar study had identified a single nucleotide polymorphism in DNMT3B which was reported to have a significant effect in colorectal cancer expression in Lynch syndrome. The effect of this polymorphism was examined in a large multinational cohort of patients however, it was found to have no effect on the age of disease onset. Several candidate polymorphisms were also identified in the DNA repair genes BRCA2, hMSH3, Lig4, hOGG1, XRCC1, XRCC2 and XRCC3 but no significant associations were identified. The results from these studies are discussed in chapters 5 and 6. All data generated from these studies were extensively analysed by a combination of statistical tests that included Kaplan-Meier survival and Cox hazard regression analysis allowing data to be stratified by both single and multi variable factors. Allele frequencies were also tested for significant deviation from the Hardy-Weinberg equilibrium, while Pearson’s Chi-square test was utilised to evaluate differences in the allele frequencies between the multinational cohort groups and distribution of genotypes. The results described in this thesis contribute to a better understanding of disease expression in Lynch syndrome as it identifies genetic factors involved in the etiology of malignancy in this disease. The progress made in this area of medical research will aid in providing better predictive information of greater accuracy regarding the risks of colorectal cancer and enable the development of personalised cancer surveillance regimens.
Terms of Use
  • Copyright 2012 Stuart Reeves
Language
  • Eng
  • eng
Related Resource
  • University of Newcastle Research Higher Degree Thesis
Identifier
  • oai:novaprd.newcastle.edu.au:uon:10691

Get this edition

  • Set up My libraries

    How do I set up "My libraries"?

    In order to set up a list of libraries that you have access to, you must first login or sign up. Then set up a personal list of libraries from your profile page by clicking on your user name at the top right of any screen.

  • All (1)
  • NSW (1)
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.
None of your libraries hold this item.

User activity


e.g. test cricket, Perth (WA), "Parkes, Henry"

Separate different tags with a comma. To include a comma in your tag, surround the tag with double quotes.

Be the first to add a tag for this edition

Be the first to add this to a list

Comments and reviews

What are comments? Add a comment

No user comments or reviews for this version

Add a comment