EVOLUTIONARY DESIGN OF GENETIC CIRCUITS AND CELL-CELL COMMUNICATIONS
CYNTHIA H. COLLINS
JARED R. LEADBETTER
FRANCES H. ARNOLD
Synthetic genetic circuits are artificial networks of transcriptional control elements inserted into living cells in order to 'program' cellular behavior. We can extend this application to programming population behavior by incorporating cell-cell communications capabilities. By designing and building such networks, cellular circuit engineers expect to gain insight into how natural genetic networks function with remarkable robustness, stability, and adaptability to changing environments. Programmed cells also have promising applications in biotechnology and medicine. A major challenge that biological circuit engineers face is the difficulty of predicting circuit performance at the design stage, with the consequence that actual construction requires significant experimental effort, even for very simple circuits. To address this fundamental obstacle we propose the use of laboratory evolution methods to create new circuit components and optimize circuit performance inside living cells.
In order to set up a list of libraries that you have access to,
you must first login
or sign up.
Then set up a personal list of libraries from your profile page by
clicking on your user name at the top right of any screen.